Lymophoprep/Ficoll separation demonstration and expected layering

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Lymophoprep/Ficoll separation demonstration and expected layering after

EFFECT OF FICOLL-HYPAQUE SEPARATION ON ACTIVATION AND DNA SYNTHESIS OF HUMAN BLOOD LYMPHOCYTES - ScienceDirect

Could anybody help me? What is the problem with my protocol of lymphocyte separation? Or our patients have got too high red blood cell number?

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