By A Mystery Man Writer
Overall, the PLBs cultured in a large 470 ml plastic vessel showed successful growth with a high growth index, high carbohydrate content, low-stress condition, and high chlorophyll content, suggesting that plastic as a culture vessel provides a good impact for culturing PLBs and has low cost. Different designs of the plant tissue culture vessel, such as size, material, and shape, may alter its microenvironment atmosphere. The present study was conducted on protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid to determine the development of PLBs on plastic and glass culture vessels of different sizes. PLBs were cultured in half-strength Murashige and Skoog (MS) medium with the same initial weight of 0.5 g in 10 replicates. The growth index of the PLBs was calculated after 11 weeks to study their growth in every vessel; additionally, biochemical analysis was performed to determine carbohydrate content, proline concentration, and photosynthesis pigments in the PLBs. Scanning electron microscopy (SEM) was performed to study stomata development on PLBs in each vessel, and histological analyses were conducted to study the cell structure. Overall, the PLBs cultured in a large 470 ml plastic vessel showed successful growth with a high growth index, high carbohydrate content, low-stress condition, and high chlorophyll content. SEM confirmed that the presence of trichome and rhizoid in PLBs cultured in the 470 ml plastic vessel. Histological analysis showed the formation of the shoot on the PLBs and the presence of starch granules. Thus, the use of plastic as a culture vessel provides a good impact for culturing PLBs and has low cost.
Horticulturae, Free Full-Text
PDF) Effects of plant growth regulators and activated charcoal on
PDF) Impact of culture vessels on micro-morphological features of
Several conditions affect and/or occur in Dendrobium tissue
PDF) A Novel Approach for Preliminary PVS2 Vitrification
Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application. - Abstract - Europe PMC
Problems Associated with Plant Micropropagation
Precocious in vitro flowering in threatened ornamental orchid, Dendrobium ovatum – Decoding the causal factors - ScienceDirect
PDF] Impact of culture vessels on micro-morphological features of
Frontiers Elicitation of the in vitro Cultures of Selected Varieties of Vigna radiata L. With Zinc Oxide and Copper Oxide Nanoparticles for Enhanced Phytochemicals Production
PDF] Impact of culture vessels on micro-morphological features of